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Department of Chemistry
The Johns Hopkins University
138 Remsen Hall
3400 N. Charles Street
Baltimore, MD 21218

Kenneth Karlin
Department Chair

Phone 410-516-7429
Fax 410-516-8420
chemdept@jhu.edu

 

Facility Instrumentation

The mass spectrometry facility at the JHU Chemistry Department contains 6 instruments equipped with a variety of different ionization techniques. Each is based on a different combination of mass analyzer design and ionization source and therefore has different analytical capabilities. Together, they form a suite of instruments that provides a comprehensive range of analytical organic mass spectral techniques. The six instruments are:

VG Analytical VG-70S Magnetic Sector Mass Spectrometer (Remsen B.13)

The VG-70S is a high resolution, double focusing magnetic sector (BE geometry) instrument with an effective mass range of 1-2100Da at full acceleration voltage and is equipped with EI and CI ionization methods. It is primarily used to provide both full scan nominal-mass spectra and accurate mass determinations of small molecules. The instrument is operated on a service basis and is located in Room B13, Remsen Hall.

VG Analytical VG-70SE Magnetic Sector Mass Spectrometer (Remsen B.13)

The VG-70SE is a high resolution, double focusing magnetic sector (BE geometry) instrument nearly identical to the VG70S instrument, but with an effective mass range of 1-2750Da at full acceleration voltage and is equipped with FAB ionization. It is primarily used to provide both full scan nominal-mass spectra and accurate mass determinations of delicate small molecules. The instrument is operated on a service basis and is located in Room B13, Remsen Hall.

Thermo Finnigan LCQ Deca Ion Trap Mass Spectrometer (Remsen B.06)

The LCQ Deca is an ion-trap based instrument normally fitted with an electrospray (ESI) ionization source. It is primarily used for analysis of bio-molecules in solution such as proteins, peptides, biopolymers and oligonucleotides. This instrument is capable of performing multi-stage ion fragmentation analyses (MSn), making it extremely useful for structural characterization of delicate bio-molecules. It can be operated on a stand-alone basis or integrated with a Finnigan Surveyor LC system for LCMS applications. Operation is on a “self-service” basis by users following training and “check-out”. This instrument is located in Room B06, Remsen Hall.

Thermo Finnigan LCQ Duo Ion Trap Mass Spectrometer (Remsen B.06)

The LCQ Duo is an ion-trap based instrument normally fitted with an electrospray (ESI) ionization source. It is primarily used for analysis of inorganic samples in solution. This instrument is capable of performing tandem mass spec ion fragmentation analyses (MS/MS), making it extremely useful for determining both parent ion masses and structural characterization of species. It is operated on a stand-alone basis and uses the same operating system as the LCQ Deca. Operation is on a “self-service” basis by users following training and “check-out”. This instrument is located in Room B06, Remsen Hall.

Shimadzu GC17A/QP5050A GCMS (Remsen B.14)

This instrument is used for routine mass spectral analysis of volatile organic mixtures. It consists of an autosampler equipped capillary GC integrally connected with an EI ionization quadrupole mass analyzer (CI is available as an option). A low polarity (5% phenyl-, 95%methyl-siloxane) column is normally installed. This instrument is also operated on a “self-service” basis by users following training and “check-out”. It is located in Room B14, Remsen Hall.

Bruker AutoFlex III MALDI-TOF/TOF (Remsen B.06)

The AutoFlex III is a state-of-the-art MALDI-TOF mass spectrometer used for qualitative analysis of high mass “delicate”  bio-molecules that cannot be analyzed by FAB or ESI-MS. It is equipped with a 355nm pulsed UV laser and can operate as a reflectron, linear or Tof/ToF instrument in both the positive and negative ionisation modes. The instrument is operated by users on a “self-service” basis following training and “check-out”, and is located in Room B06, Remsen Hall.

Thermo Finnigan Surveyor / LCQ Fleet LC-MS System (Remsen B14)

The Surveyor / LCQ Fleet is an electrospray equipped ion-trap based LC-MS used for routine seperation and analysis of biomolecules. The LC includes an auto-sampler for automated multi sample analysis, solvent blending capabilities for gradient analyses, a temperature controlled column compartment and a photodiode detector for spectroscopic detection of components to complement the mass spectral detection. The instrument is normally equipped with a reverse phase C 18 column and Water, Methanol and Acetontrile solvents. The mass spectrometer is capable of performing multi-stage ion fragmentation analyses (MSn). Operation is on a “self-service” basis by users following training and “check-out”. This instrument is located in Room B14, Remsen Hall.

Waters Acquity / Xevo-G2 UPLC-MS system (Remsen B.14)

The Acquity / XevoG2 system combines a high pressure (~15000 psi) UPLC system with a high mass resolution Q-ToF MS/MS instrument. It is used for more specialist seperation and analysis of biomolecules. The LC includes an auto-sampler for automated multi sample analysis, the ability to blend up to four seperate solvents stream, multiple column accomodations, temperature controlled column compartments and a photodiode detector for UV spectroscopic detection of components to complement the mass spectral detection. The instrument is usually supplied with Water, Methanol and Acetontrile solvents. The mass spectrometer is capable of performing high resoluuion MS and MS/MS analyses. Operation is on a “self-service” basis by users following training and “check-out”. This instrument is located in Room B14, Remsen Hall.